lab-protocols

20X Sodium Borate Buffer (SB Buffer)

Reagents

• Sodium tetraborate decahydrate (CAS 1303-96-4)
  • 381.37 g/mol

Concentrations

• 100 mM Sodium tetraborate decahydrate

Procedure

Per liter

1. Dissolve 38.14 g Sodium tetraborate decahydrate in 800 mL of pure nuclease free water
2. Bring volume to 1 L with pure nuclease free water

Comments

May want to confirm that this produces a 20X solution with a pH of 8.0 but probably isn’t necessary

Sources

Brody and Kem 2004. Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis. Link