20X Sodium Borate Buffer (SB Buffer)
Reagents
- Sodium tetraborate decahydrate (CAS 1303-96-4)
Concentrations
- 100 mM Sodium tetraborate decahydrate
Procedure
Per liter
- Dissolve 38.14 g Sodium tetraborate decahydrate in 800 mL of pure nuclease free water
- Bring volume to 1 L with pure nuclease free water
May want to confirm that this produces a 20X solution with a pH of 8.0 but probably isn’t necessary
Sources
Brody and Kem 2004. Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis. Link