lab-protocols

Proteinase K Buffer Solution

Reagents

• 1 M Tris-HCl Buffer Solution, pH 8.0
• 0.5 M EDTA Solution, pH 8.0
• 10% (m/v) SDS Solution

Final concentrations

• 10 mM Tris-HCl
• 10 mM EDTA
• 1% (m/v) SDS

Procedure

Per 100mL

1. Add 1 mL 1 M Tris-Cl solution
2. Add 2 mL 0.5M EDTA solution
3. Add 10 mL 10% (m/v) SDS solution
4. Bring volume to 100 mL with 87 mL of pure nuclease free water