lab-protocols

Protocols used in the Phyletica lab at Auburn University. View Github repository.

Extractions

• Phenol Chloroform Extraction Protocol
• SPRI Bead Extraction Protocol

PCR

• Promega GoTaq PCR
• KAPA HiFi PCR

RADSeq

• 3RAD Protocol
• Adapters

Other

• Gel Electrophoresis Protocol
• Ethanol Precipitation Protocol

Solutions

• EDTA Solution
• Proteinase K Buffer Solution
• Sodium Borate Buffer Solution
• SDS Solution
• TLE Buffer Solution
• Tris-HCl Buffer Solution
• 5M NaCl Solution